Coding

Part:BBa_K317999:Design

Designed by: Daisuke HIRAOKA   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)


RhlB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 904
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 381


Design Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication


Source

Pseudomonas aeruginosa

References